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Genera introduce from the ≥0.001% relative wealth inside V1–V3 and you may/or V4 datasets are believed. Genera which have less than doubled difference in relative variety between your two primer establishes receive with grey groups, and people who is overrepresented by at least twofold that have one to of the primer set get inside the red-colored (V4) and you will blue (V1–V3). The brand new twofold distinction is actually an arbitrary options; but not, they describes the newest suspicion i constantly come across inside amplicon investigation. Genus names receive for everybody taxa establish at the very least out of 0.1% mean relative abundance (excluding people with de novo brands).
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